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By dosage, the mean changes were: 500 mcg: 127% 750 mcg: 176% (single study) 1,000 mcg: 280% 1,500 mcg: 310% 2000 mcg: 260% 5,000 mcg: 62% While 1,500 mcg of vitamin B12 showed the highest average increase, the effect plateaued or declined at higher doses, suggesting a possible saturation of absorption pathways

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After centrifugation at 1000g for 15 min, the serum levels of proBDNF and BDNF were determined using enzyme-linked immunosorbent assay (ELISA) performed on 96-well plates pre-coated with highly specific monoclonal antibodies

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